The application of thermoelectric cooling technology in PCR instruments

The application of thermoelectric cooling technology in PCR instruments

The application of thermoelectric cooling technology in PCR instruments mainly lies in temperature control. Its core advantage is the rapid and precise temperature control capability, which ensures the success rate of DNA amplification experiments.

Key application scenarios

1. Precise temperature control

The PCR instrument needs to cycle through three stages: high-temperature denaturation (90-95℃), low-temperature annealing (55-65℃), and optimal temperature extension (70-75℃). Traditional refrigeration methods are difficult to meet the accuracy requirement of ±0.1℃. Thermoelectric cooling ,peltier cooling technology achieves millisecond-level temperature regulation through the Peltier effect, avoiding amplification failure caused by a 2℃ temperature difference.

2. Rapid cooling and heating

Thermoelectric cooling modules, thermoelectric modules, peltier devices, peltier modules can achieve a cooling rate of 3 to 5 degrees Celsius per second, significantly shortening the experimental cycle compared to the 2 degrees Celsius per second of traditional compressors. For instance, the 96-well PCR instrument adopts zonal temperature control technology to ensure consistent temperatures in all well positions and avoid a 2℃ temperature difference caused by edge effects.

3. Enhance the reliability of equipment

The thermoelectric cooling modules,peltier modules ,peltier lements,TEC modules of Beijing Huimao Cooling Equipment Co., Ltd. have become the core temperature control components of PCR instruments due to their high reliability. Its small size and noise-free features make it suitable for the precision requirements of medical equipment.

Typical application cases

96-well fluorescence quantitative PCR detector: Integrated with a thermoelectric cooling module,TEC module ,peltier device,peltier modules it enables precise temperature control of high-throughput samples and is widely used in fields such as gene expression analysis and pathogen detection.

Portable medical refrigerators: thermoelectric cooling ,peltier cooling portable medical refrigerators used to store products such as vaccines and medicines that require a low-temperature environment, ensuring temperature stability during transportation.

Laser treatment equipment:

Thermoelectric cooling modules, peltier elements, thermoelectric modules cool the laser emitter to reduce the risk of skin burns and enhance treatment safety.

TEC1-39109T200 Specification

Hot side temperature is 30 C,

Imax： 9A

Umax: 46V

Qmax：246.3W

ACR: 4±0.1Ω（Ta= 23 C）

Delta T max： 67 -69C

Size ： 55x55x3.5-3.6mm

TES1-15809T200 Specification

Hot side temperature: 30 C,

Imax ： 9.2A，

Umax: 18.6V

Qmax：99.5 W

Delta T max： 67 C

ACR：1.7 ±15% Ω （1.53 to 1.87 Ohm）

Size  ： 77×16.8×2.8mm

Wire: 18 AWG silicone wire or equal Sn-plated on the surface, high temperature Resistance 200℃